Biobanking of cryopreserved primary CLL cells, plasma, and serum.
Isolation of B cells, T cells, and monocytes/macrophages from peripheral blood (AutoMACS NEO Separator, Miltenyi Biotec).
Isolation of cells from human and murine tissues (GentleMACS Tissue Dissociator, Miltenyi Biotec).
2D and 3D (spheroid) culture of primary CLL PBMCs and feeder co-cultures.
Flow cytometry for immunophenotyping, cell cycle, secretion, cytotoxicity, and proliferation (Accuri C6 Plus, Becton Dickinson)
Nucleic acid quantification, ELISA, and reporter assays (Infinite 200 plate reader, Tecan)
Cell genetic manipulation by plasmid overexpression and siRNA-mediated gene silencing (4D-Nucleofector, Lonza)
Fluorescence and bright-field microscopy (EVOS M5000, Invitrogen)
Real-time PCR for DNA/RNA detection and quantification (LightCycler 480 II, Roche)
Protein expression analysis by SDS-PAGE and Western blot (Mini-PROTEAN and Trans-Blot Turbo systems, Bio-rad)